Category: HPLC Primer
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10mM Ammonium Acetate Buffer 90:10 in Acetonitrile Preparation for HPLC and LCMS - Tips & Suggestions
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Aqueous Normal Phase ANP Ballistic Gradients Compared to Same in HILIC - Tips and Suggestions
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Broad Peaks That are Non Symmetrical in LCMS in Reversed Phase Solutions - Troubleshooting
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Citric acid retention and peak shape has changed compared to data from a previous run what happened - FAQ
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Direct Silicon to Carbon Bonds on Cogent TYPE-C HPLC HPLC Columns Produce Longer Column Lifetimes - Tech Information
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Do Cogent TYPE-C Silica columns have a partial positive charge and if so is retention for basic compounds reduced because of this - Tech Information
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Effective Filtration Area and Burst Pressure for AQ Brand Syringe Filters - Tech Information
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Effects of Washing and Re-using Autosampler Vials on Sample Loss and Contamination - Tech Information
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Explanation of Wavelength and Absorbance Precision Test of the UV-vis Software -Tech Information
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Extra Column Volume calculation using the Chemical Solutions Qualification Kit software - How To
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FDA Approval is not Available for RSA Vials or for Use in Clinical Resting - Tech Information
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For Sugars Analyzed by LCMS Sodium Acetate is recommended for Sensitivity of Na Adducts - Tips & Suggestions
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Formic Acid Improves Peak Shapes Retention and Carryover for Polar Compounds with the Cogent Diamond Hydride HPLC Column - Tips & Suggestions
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How Do the Terms Nano Capillary and Micro LC Differ in Terms of Column ID and Typical Flow Rates - HPLC Primer
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How Does Ammonium Fluoride Affect HPLC and LCMS Data if using Aqueous Normal Phase ANP Methods - HPLC Primer
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How Tight Should an Autosampler Vial Cap be to Prevent Push Through of the Septa During Injection - HPLC Primer
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I heard that there is not a good alternative to HILIC to achieve sufficient retention for some compounds - FAQ
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I see a strange saw tooth type of pattern in the UV baseline using my Cogent HPLC column what is it and how can I fix it - FAQ
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If an analyte is only soluble at basic pH can you use a basic mobile phase for my method - FAQ
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In a Cap that Contains a Butyl Rubber PTFE Septa Which Direction does the PTFE Membrane Face - HPLC Primer
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Ion Content Differences Between Clear and Amber Borosilicate Glass Vials – Tech Information
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Is there a Difference in the Properties of Different Colors of Rubber in Autosampler Vial Cap Septa - HPLC Primer
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LCMS Challenges with HILIC Mobile Phases from Salt Buildup and Ion Suppression Explained - Tips & Suggestions
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LFER Studies Compared to Log D Data in Describing the Aqueous Normal Phase ANP Retention - Tips & Suggestions
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Ligand Bonding to Silica Hydride Particles in the Cogent UDA HPLC columns - Tech Information
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Literature References | Bibliography for Cogent TYPE-C HPLC Publications - Tech Information
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Lower Retention When Functional Group in a Compound Should be Ionized - Troubleshooting Tips
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Moisture Sealing Guidance when Using Autosampler Vials and Caps for HPLC and GC - Tech Information
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My retention times are changing after storing my buffer and what could be causing this and tips to help avoid issues - FAQ
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PEEK HPLC Fittings Do Not Fit All Receiving Ports - Compatibility Rules and Pressure Tips - HPLC Primer
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Positive or Negative ion mode Salt Suggestions for LC-MS Method Development - Tips & Sugeestions
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Pre‑Cut Stainless Steel Tubing for HPLC: Burr‑Free, Zero‑Dead‑Volume Connections - Tech Information
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Prepare a 16mM Ammonium Acetate Buffer 90:10 in Acetonitrile for HPLC - Tips & Suggestions
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RSA Autosampler Vials Compared to Silanized Vials for Pharma Test Applications - HPLC Primer
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Since Cogent TYPE-C Silica columns use a transition metal catalyst in production what is the presence of any trace quantity on the surface of the stationary phase- FAQ
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Specifications of Different Grades of Stainless Steel Used in HPLC Columns - Tech Information
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Stainless‑Steel Ferrules and Stainless‑Steel and PEEK Hybrid Ferrules Differences - HPLC Primer
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Tanaka Plots Do Not Apply to Columns Used with Aqueous Normal Phase Methods - Tech Information
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To obtain greater sensitivity than Na adducts for sugars could you add ammonia NH4 instead - FAQ
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Troubleshooting Problems With Poor HPLC Results Before Examining the Column - Tips & Suggestions
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TYPE-C Silica Is Made with High Purity Silica and Has a Low Metal Content - Tech Information
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Using Silica or Silica‑C Guard Columns with Analytical Columns of Different Phases - HPLC Primer
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Vials Listed as 12 x 32 mm Compatibility with Autosamplers' Maximum Diameter of 11.8 mm - HPLC Primer
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What are the Differences Between 33 and 51 Expansion Glass and Which Should I Choose - HPLC Primer
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What are the Temperature and Pressure Limits for Amber Glass Autosampler Vials - Tech Information
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What are the typical flow rates recommended by HPLC column companies to start with for various column ID - Primer
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What is the difference between related compounds and related substances in pharmaceutical HPLC - FAQ
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What is the effect of extra column volume for different HPLC instruments on the same method - FAQ
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What is the Functional Difference Between the MRQ and the Max Recovery Autosampler Vials - HPLC Primer
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When I run gradients on my HPLC and switch from one gradient to another my baseline shows as negative and if I auto zero it becomes positive for the remainder of the run but when I start again it is negative - Primer
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Why are ionized polar compounds generally better retained in Aqueous Normal Phase ANP HPLC - FAQ
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