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Category: HPLC Primer
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Adjusted retention time hold up time - Primer
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Aqueous Normal Phase ANP defined & compared to Reversed Phase & Normal Phase definitions HPLC - Tech Information
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Calculate the ligand density of a bonded phase of an HPLC column - How To
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Calculating theoretical plates N at half height - How To
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Choose an appropriate guard column when using a Cogent TYPE-C HPLC column - How To
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Column ID Considerations for LCMS Applications - Tech Information
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Determine dwell volume of gradient HPLC systems - How To
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Determine limits of detection LOD and limits of quantification LOQ - How To
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Determine pore volume of an HPLC column using HPLC injections - How To
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Determine the asymmetry factor in HPLC at 10 percent peak height - How To
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Determining HPLC method accuracy - How To
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Determining the precision of an HPLC method - Tips & Suggestions
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Difference between adsorption and absorption - Primer
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Difference between assay and purity regarding pharmaceutical analysis - Primer
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Do Cogent TYPE-C Silica columns have a partial positive charge and if so is retention for basic compounds reduced because of this - Tech Information
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Dwell volume effect on HPLC analyte retention in gradient methods - Primer
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Establishing HPLC method linearity - Tips & Suggestions
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Explanation of Wavelength and Absorbance Precision Test of the UV-vis Software -Tech Information
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Extra Column Volume calculation using the Chemical Solutions Qualification Kit software - How To
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Factors that affect sensitivity in HPLC - Tech Information
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Fractions to decimals to metric chart - Tech Information
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Generic variation of Teflon and uses in plastic lab tubing - Primer
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How can I improve accuracy and precision when separating polar compounds by HPLC using Aqueous Normal Phase ANP - FAQ
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How can sample concentration have an effect on peak shape in HPLC - FAQ
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How is pressure inside tubing calculated for HPLC - FAQ
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How Pulsations from an HPLC Pump Can Impact Column Performance - Tech Information
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How to back flush and wash an HPLC column for reuse - Tips & Suggestions
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How to determine concentration of an impurity in HPLC with the relative response factor RRF - How to
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How to prepare 10mM ammonium acetate buffer 90:10 in acetonitrile for HPLC and LCMS - Tips & Suggestions
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How to prepare 16mM ammonium acetate buffer 90:10 in acetonitrile for HPLC - Tips & Suggestions
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How to tighten screw caps properly - Tips & Suggestions
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I heard that there is not a good alternative to HILIC to achieve sufficient retention for some compounds - FAQ
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If you are not seeing an expected peak in an HPLC assay - Tips & Suggestions
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Improvement in signal to noise in LCMS with an ANP method - Tips & Suggestions
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Improving peak shapes in an HPLC method in Aqueous Normal Phase ANP - Tips & Suggestions
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Integrate a peak with a shoulder - How to
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Interpreting mass spectral data - Primer
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Know the void volume of an HPLC column - Tips & Suggestions
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Know when to discard an outlier data point in HPLC by using the Q Test - How To
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Measure signal to noise ratio in an HPLC chromatogram - How to
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Measuring pump flow accuracy in HPLC - How to
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Missing peaks using UV detection in HPLC methods - Tech Information
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Octanol Water Partition Coefficient use in HPLC - Primer
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Our glossary of terms used in separation science - Primer
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Physical nature of PEEK frits - Technical Information
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pK Values of common chemical functional groups in HPLC - PRIMER
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Poor HPLC peak shape can be due to injection sample mismatch - Tips & Suggestions
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Pre mix your diluent and mobile phase in sample preparation for HPLC - Tips & Suggestions
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Quaternary amines analysis by LCM and finding the right ion - How To
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Relative response factor RRF and correction factor - How To
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Reliability of wet chemical assays versus HPLC assays - Primer
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Sample concentration changes can effect retention time in HPLC - Primer
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Terminology and Criteria for Chirality - Primer
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The common names of HPLC column diameters - Primer
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The difference between potency and purity in drug products - Tips & Suggestions
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Theoretical plate estimates indicates column condition - Primer
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Theoretical plate N estimate for real samples - How to
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Types of Chemical Isomers and Recommended HPLC Columns for Effective Separation - Tips & Suggestions
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Use a linear flow rate test to check back pressure on an HPLC column - Tech Information
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Use Teflon bottles for A and B solvents in negative ion mode LCMS - Primer
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Validating HPLC and LC-MS Methods for Regulated Labs - Primer
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What are the maximum changes allowed in a USP method- FAQ
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What are the typical flow rates recommended by HPLC column companies to start with for various column ID - Primer
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What are the uses of PEEK HPLC column hardware - FAQ
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What are theoretical plates and HETP in HPLC - Primer
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What can I use for a Reference Solution against Holmium Oxide - FAQ
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What determines the retention of a molecule in reversed phase HPLC - FAQ
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What does residual volume mean with autosampler vials - FAQ
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What does the Specification 9-425mm mean for Screw Top Autosampler Vials - FAQ
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What is 0.1 % w/v ammonium acetate in terms of molarity - FAQ
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What is a pyrogen - FAQ
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What is a steady state for surfaces like syringe filters - FAQ
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What is a system peak as defined for HPLC - Primer
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What is pKa and how is it related to pH in HPLC mobile phases and sample diluents - FAQ
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What is Post Time in an HPLC gradient method - Primer
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What is TFA and when should I use it - Primer
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What is the difference between a Waters type fitting and a standard 10-32 fitting - FAQ
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What is the difference between OQ and PQ- FAQ
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What is the difference between related compounds and impurities - FAQ
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What is the difference between related compounds and related substances in pharmaceutical HPLC - FAQ
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What is the difference between Verifications Validation and Qualification - PRIMER
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What Is the Difference Between Weak and Strong Cation Exchange in Ion Exchange Chromatography - FAQ
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What is the effect of extra column volume for different HPLC instruments on the same method - FAQ
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What is the useable volume in 2mL autosampler vials - FAQ
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What makes glass borosilicate glass - Tech Information
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When I run gradients on my HPLC and switch from one gradient to another my baseline shows as negative and if I auto zero it becomes positive for the remainder of the run but when I start again it is negative - Primer
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Why is isopropanol rarely used as a solvent in HPLC or LCMS methods - FAQ
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Why is Nylon called Nylon - FAQ
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Will phosphates precipitate in an HPLC column - PRIMER
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