Category: HPLC Primer
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Aqueous Normal Phase defined & compared to Reversed Phase & Normal Phase definitions HPLC - Tech Information
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Causes of high baseline noise with Cogent Diamond Hydride HPLC column - Tips & Suggestions
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Citric acid retention and peak shape has changed compared to data from a previous run what happened - FAQ
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Direct Silicon to Carbon Bonds on Cogent TYPE-C HPLC HPLC Columns Produce Longer Column Lifetimes - Tech Information
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Do Cogent TYPE-C Silica columns have a partial positive charge and if so is retention for basic compounds reduced because of this - Tech Information
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Effective Filtration Area and Burst Pressure for AQ Brand Syringe Filters - Tech Information
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Effects of Washing and Re-using Autosampler Vials on Sample Loss and Contamination - Tech Information
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Explanation of Wavelength and Absorbance Precision Test of the UV-vis Software -Tech Information
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Extra Column Volume calculation using the Chemical Solutions Qualification Kit software - How To
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For Sugars Analyzed by LCMS Sodium Acetate is recommended for Sensitivity of Na Adducts - Tips & Suggestions
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For the separation of polar intermediates in reversed phase HPLC which C18 column do you recommend - FAQ
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How do RSA Autosampler Vials Compare Against Silanized Vials for Pharma Applications - HPLC Primer
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How Do the Terms Nano Capillary and Micro LC Differ in Terms of Column ID and Typical Flow Rates - HPLC Primer
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How Does Ammonium Fluoride Affect HPLC and LCMS Data if using Aqueous Normal Phase ANP Methods - HPLC Primer
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How Tight Should an Autosampler Vial Cap be to Prevent Push Through of the Septa During Injection - HPLC Primer
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How to determine concentration of an impurity in HPLC with the relative response factor RRF - How to
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How to prepare 10mM ammonium acetate buffer 90:10 in acetonitrile for HPLC and LCMS - Tips & Suggestions
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How to prepare 16mM ammonium acetate buffer 90:10 in acetonitrile for HPLC - Tips & Suggestions
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I heard that there is not a good alternative to HILIC to achieve sufficient retention for some compounds - FAQ
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I see a strange saw tooth type of pattern in the UV baseline using my Cogent HPLC column what is it and how can I fix it - FAQ
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If an analyte is only soluble at basic pH can you use a basic mobile phase for my method - FAQ
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In a Cap that Contains a Butyl Rubber PTFE Septa Which Direction does the PTFE Membrane Face - HPLC Primer
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Is there a Difference in Performance Between Clear and Amber RSA Autosampler Vials - HPLC Primer
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Is there a Difference in the Properties of Different Colors of Rubber in Autosampler Vial Cap Septa - HPLC Primer
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Metal free Dursan Coated Stainless Steel HPLC Columns Comparison to PEEK Column Performance - Tips & Suggestions
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My retention times are changing after storing my buffer and what could be causing this and tips to help avoid issues - FAQ
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Since Cogent TYPE-C Silica columns use a transition metal catalyst in production what is the presence of any trace quantity on the surface of the stationary phase- FAQ
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The Advantage or Disadvantage of Using Certified Autosampler Vials Compared to MICROSOLV Brand Vials - Tips & Suggestions
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To obtain greater sensitivity than Na adducts for sugars could you add ammonia NH4 instead - FAQ
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Types of Chemical Isomers and Recommended HPLC Columns for Effective Separation - Tips & Suggestions
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Using different Cogent HPLC stationary phases for different Selectivity - Tech Information
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Vials are Listed as 12 x 32 mm but my Autosampler Accepts a Maximum Diameter of 11.8 mm Will They Fit - HPLC Primer
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What are the Advantages and disadvantages of Acid Washing RSA Autosampler Vials - HPLC Primer
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What are the Differences Between 33 and 51 Expansion Glass and Which Should I Choose - HPLC Primer
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What are the Temperature and Pressure Limits for Amber Glass Autosampler Vials - Tech Information
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What are the typical flow rates recommended by HPLC column companies to start with for various column ID - Primer
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What Can Compromise the Surface Treatment of Silanized Glass Autosampler Vials - HPLC Primer
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What is the difference between related compounds and related substances in pharmaceutical HPLC - FAQ
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What Is the Difference Between Weak and Strong Cation Exchange in Ion Exchange Chromatography - FAQ
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What is the effect of extra column volume for different HPLC instruments on the same method - FAQ
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What is the Functional Difference Between the MRQ and the Max Recovery Autosampler Vials - HPLC Primer
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What is the maximum concentration of ammonium fluoride to use in a mobile phase to avoid column damage - Tips & Suggestions
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When I run gradients on my HPLC and switch from one gradient to another my baseline shows as negative and if I auto zero it becomes positive for the remainder of the run but when I start again it is negative - Primer
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Which Crimp Caps and Septa are Suitable for Use with Solid Phase Micro Extraction SPME Vials - FAQ
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Which is better to Use Snap Top or Screw Top Autosampler Vials and Caps for Preventing Evaporation - FAQ
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Why are ionized polar compounds generally better retained in Aqueous Normal Phase ANP HPLC - FAQ
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Why are Pipettors not Recommended for Dispensing Non Aqueous Liquids Accurately and Precisely - FAQ
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